J Integr Plant Biol. ›› 2025, Vol. 67 ›› Issue (1): 7-10.DOI: 10.1111/jipb.13797 cstr: 32098.14.jipb.13797
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Efficient gene disruption in polyploid genome by Cas9–Trex2 fusion protein
Wenbo Pan1,2†, Chunlei Gao1†, De Niu1†, Jinghua Cheng1, Jiao Zhang1,3, Xiying Yan1, Qiang Long1, YaoYao Zhu1,3, Wenjing Sun1, Qi Xie4, Yuehui He1, Xing Wang Deng1, Huawei Zhang1* and Jian Li1*
- 1. State Key Laboratory of Wheat Improvement, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Peking University Institute of Advanced Agricultural Sciences, Weifang 261325, China
2. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Ministry of Education & Guangdong Provincial Key Laboratory of Laser Life Science, School of Life Sciences, South China Normal University, Guangzhou 510631, China
3. Shenzhen Tidy field System Biotechnology Co., Ltd, Shenzhen 518107, China
4. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China
†These authors contributed equally to the work.
*Correspondences: Huawei Zhang (huawei.zhang@pku-iaas.edu.cn); Jian Li (jian.li@pku-iaas.edu.cn, Dr. Li is fully responsible for the distribution of the materials associated with this article).
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Received:2024-01-25
Accepted:2024-10-05
Online:2024-10-25
Published:2025-01-01
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Supported by:This work was supported by the Natural Science Foundation of Shandong Province (ZR2021ZD30, ZR2022ZD22), the Key R&D Program of Shandong Province (ZR202211070163), Taishan Scholar Foundation of Shandong Province (TSQN202103160), and the Excellent Youth Foundation of Shandong Scientific Committee (ZR202103010168).
