Abstract: RNA interference (RNAi) is increasingly used for plant protection against pathogens and pests. However, the traditional delivery method causes plant tissue damage, is affected by environmental factors, and faces difficulties in penetrating the barriers of cell walls and the limitations of plant species, ultimately leading to low delivery efficiency. With advances in nanotechnology, nanomaterials (NMs) have been identified as effective carriers for nucleic acid delivery because of their ability to operate independently of external mechanical forces, prevent degradation by bioenzymes, exhibit good biocompatibility, and offer high loading capacity. This review summarizes the application of NM-mediated RNAi against plant pathogens and pests, focusing on how different NMs break through the cell barriers of plants, pathogens, and pests according to their size, morphology, and charge characteristics. Furthermore, we discuss the advantages and improvement strategies of NMs as nucleic acid delivery carriers, alongside assessing their potential application for the management of plant pathogens and pests.

Key words: dsRNA, nanomaterials, nucleic acid delivery, plant protection, RNAi, siRNA