J Integr Plant Biol.

• Review Article • Previous Articles    

Phytosterols: Structural variations, biosynthetic pathways, and their biological roles

Chao Tan and Shuzhen Men*   

  1. Tianjin Key Laboratory of Protein Sciences, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University,Tianjin 300071, China

    *Correspondence: Shuzhen Men (shuzhenmen@nankai.edu.cn)

  • Received:2025-03-31 Accepted:2025-12-14 Online:2026-01-08
  • Supported by:
    This research was ?nancially supported by the National Natural Science Foundation of China (32370271, 32070281,and 31870230 to SM), General Program of Natural Scicence Foundation of Tianjin (23JCYBJC01180), and Key Research and Development Program of Tianjin (24YFXTHZ00050).

Abstract: Phytosterols are a diverse class of isoprenoid-derived lipids that serve as essential structural components of plant membranes and regulators of growth and reproduction. Unlike animals and fungi, which predominantly utilize cholesterol and ergosterol, plants produce a complex array of over 250 sterol molecules. These include major forms such as β-sitosterol, stigmasterol, and campesterol as well as minor components like cholesterol and various sterol biosynthetic intermediates. This review provides a comprehensive overview of plant sterols, first addressing their structural diversity and distribution across species and tissues, and then exploring their biosynthesis, transport, and functions. A key focus is placed on their role as membrane modulators, influencing fluidity, permeability, and the formation of lipid rafts. Finally, we synthesize genetic and molecular evidence, demonstrating the critical functions of sterols and their derivatives in both reproductive and vegetative development. We conclude by highlighting persistent gaps in our knowledge and proposing future research directions to unravel the multifaceted roles of these essential molecules.

Key words: conjugated sterols, embryogenesis, lipid raft, phy-tosterols, reproductive development, sterol biosynthesis, vegetative development

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