J Integr Plant Biol. ›› 1993, Vol. 35 ›› Issue (9): -.
• Research Articles •
Xia Guang-min, Li Zhong-yi and Chen Hui-min
Abstract: Heracleum moellendorffiz Hance is a herb belonging to Umbelliferae used in traditional medicine in China. The young stem-nodes were induced for callus formation on MS medium containing 1 mg/L 2,4-D. After subcultured for about five months, the embryogenic calli were used for cell suspension culture. The protoplasts were prepared from this suspension by digestion with enzyme mixture containing 1. 5% cellulase Onozuka R-10 +0. 3% macerozyme R-10 + 0. 5% snailase + 5 mmol CaCl2 + 0. 6 mol/L mannitol, at pH 5.8, and cultured in modified MS and modified N6 media with 0.3 % agarose. They divided after 3 days and developed into small cell colonies after about 2 weeks. From this time on, the glucose concentration in the culture media was decreased to 0. 2 mol/L,which led to futher growth of the colonies to small calf . After a period of proliferation on solid medium with 0. 5 mg/L 2,4-D, the calli were transferred to a medium with 0. 1 mg/L zeatin on which somatic embryos differentiated and developed to plantlets
Key words: Heracleum moellendorffiz Hance, Suspension cell, Protoplast culture, Plant regeneration
Xia Guang-min, Li Zhong-yi and Chen Hui-min. Plant Regeneration from Cell Supension Derived Protoplasts of Heracleum moellendorffii[J]. J Integr Plant Biol., 1993, 35(9): -.
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