J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (2): 122-130.DOI: 10.1111/jipb.12000

• New Technology •     Next Articles

An Efficient Rice Mutagenesis System based on Suspension-Cultured Cells

Yuan-Ling Chen, Hui-Lin Liang, Xing-Liang Ma, Su-Lin Lou, Yong-Yao Xie, Zhen-Lan Liu, Letian Chen and Yao-Guang Liu*   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
  • Received:2012-07-25 Accepted:2012-10-15 Published:2013-02-01
  • About author:*Corresponding author Tel: +86 20 8528 1908; Fax: +86 20 8528 2180; E-mail: ygliu@scau.edu.cn

Abstract:

Plant mutants are important bio-resources for crop breeding and gene functional studies. Conventional methods for generating mutant libraries by mutagenesis of seeds with physical or chemical agents are of low efficiency. Here, we developed a highly-efficient ethyl methanesulfonate (EMS) mutagenesis system based on suspension-cultured cells, with rice (Oryza sativa L.) as an example. We show that treatment of suspension-cultured tiny cell clusters with 0.4% EMS for 18–22 h followed by differentiation and regeneration produced as high as 29.4% independent mutant lines with visible phenotypic variations, including a number of important agronomic traits such as grain size, panicle size, grain or panicle shape, tiller number and angle, heading date, male sterility, and disease sensitivity. No mosaic mutant was observed in the mutant lines tested. In this mutant library, we obtained a mutant with an abnormally elongated uppermost internode. Sequencing and functional analysis revealed that this is a new allelic mutant of eui (elongated uppermost internode) caused by two point mutations in the first exon of the EUI gene, representing a successful example of this mutagenesis system.

Chen YL, Liang HL, Ma XL, Lou SL, Xie YY, Liu ZL, Chen LT, Liu YG (2013) An efficient rice mutagenesis system based on suspension-cultured cells. J. Integr. Plant Biol. 55(2), 122–130.

Key words: Rice;mutagenesis, EMS, suspension cell culture, mutant

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22