J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (11): -.

• Research Articles •    

Influence of Nitrogen Sources on Glutamine Synthetase in Wheat Seedling

Huang Qin-ni, Yin Li-ping, Chai Xiao-qing, Liu Xiang-lin, Hong Jian-ming and Zhao Wei-ping   

Abstract: Influence of nitrogen sources on the activity of glutamine synthetase (GS), GS isozymes and GS-mRNA in wheat (Triticum aestivum L. ) seedlings has been studied by DEAE-cellu- lose column chromatography, enzyme activity assay and Northern blot technique, and activity of GS and nitrate reductase (NR) was compared. The results indicated that the activity of GS in roots (GSr) and cytoplasmic GS (GS1) in both intact and excised leaves was higher with NH4+-N as the sole nitrogen source than with NO3--N. However, NO3- promated the activity of chloroplasmic GS (GS2) in leaves of wheat with NO3- -N as the sole nitrogen surce. On the level of transcription, NH+ raised the level of GS mRNA in roots, but NO3- raised the level of GS-mRNA in leaves of wheat seedling.

Key words: Glutamine synthetase, Nitrogen source, Glutamine synthetase-mRNA, Wheat

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q