J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (7): -.

• Research Articles •    

Plant Regeneration of Protoplasts of Trifolium lupinaster L. from Cell Suspension Cultures

Zhao Gui-lan, Luo Xi-ming and Liu Yan-zhi   

Abstract: paper deals with regeneration of protoplasts in cell suspension cultures of hypocothl from Trifolium lupinaster L. on the SL2 basal medium with BA 0.1 mg/L and picloram 0.06 mg/L for 3--4 month,s. The protopiasts were isolated from suspensions cells subcultured for 3 days and were recuhured in modified liguid medium 8p. The first division of the regenerated cell occurred 3 days after being cultured in medium Bp. Small calli could be seen with naked eyes by one month. The calli when grew up to 2 mm long, were transferred in succession differentiation medium A and B for organ differentiation. The differentiated shoots formed their roots on 1/2 MS supplamented with NAA 1.0mg/L and then grew into plantlets.

Key words: Trifolium lupinaste, Protoplast, Plant regeneration

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