Abstract: Anthers of "Jinghua No. 1" winter wheat were cultured on N6 basal medium supplemented with 2,4-D 2 mg/L, KT 0.5 mg/L and NaC1 of various concentrations for defining selection stress. Irradiated and non-irradiated anthers were cultured on the medium salinized with 0.3%, 0.4% or 0.5% NaC1 for callus induction. Then screened calli for differentiation were transferred to medium containing NaC1 with the same concentrations as that for callus induction. 6 pollen plants were selected in which 1 pollen plant was obtained from irradiated anthers on the medium containing 0.5% NaCl. Only one seed was collected from this pollen plant. Growth status in the γH3 of the above-screened pollen plant was normal in the moderately salt-affected soil. It was demonstrated that salttolerance of 3 strains in the γH4 upon further valuation of water culture, salt-soil tank and field plotting was significantly higher than that of parent.

Key words: Wheat, Anther culture, Salt-tolerant variant