J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (1): -.

• Research Articles •    

Cell Wall Regenration During Early Development of Mesophyll Protoplasts of Astragalus melilotoides var. tenuis

Li Xi-wen, An Li-jia, Tong De-juan, Huang Bai-qu and Hao Shui   

Abstract: The techniques of transmission electron microscopy (TEM), electron microscopy (EM) cytochemical visualization of polysaccharide, cell wall flourescence labelling of cell wall and inhibition of wall formation by coumarin treatment were used to explore the cell wall regeneration and its chemical characteristics in mesophyll protoplasts of Astragalus melilotoides var. tenuis. The results showed that after 24 h in culture a number of protruding vesicles, as well as a small amount of fibrillar component were formed on the surface of protoplasts. On day 3, the amount of fibrils increased significantly. On day 5, regenerated primary wall composed of fibrils and granules were observed, in which polysacchaides were detected as result of the periodic acid-silver methenamine reaction. In addition, after 36 h in culture, the protoplasts tended to coalesce, flourescence staining and coumarin treatment demonstrated that the protoplast adhesion was the result of cell wall formation. Based on these data, problems such as the structure of regenerated wall and its chemical nature, etc. were discussed.

Key words: Mesophyll protoplast, Cell wall regeneration, Cytochemistry of polysaccharide, Protoplast adhesion, Astragalus melilotoides var. tenuis

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