Downregulation of the Basic Peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid

doi:10.1111/j.1744-7909.2010.00888.x

J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (2): 244-251.DOI: 10.1111/j.1744-7909.2010.00888.x cstr: 32098.14.j.1744-7909.2010.00888.x

• Research Articles • Previous Articles

Downregulation of the Basic Peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid

María Josefa López Núñez-Flores¹, Jorge Gutiérrez ², Laura V. Gómez-Ros ¹, Esther Novo Uzal², Mariana Sottomayor³ and Alfonso Ros Barceló

^{1* 1}Department of Plant Biology, University of Murcia, E-30100, Murcia, Spain

Received:2009-08-27 Accepted:2009-09-11 Published:2010-02-01
About author: ²Department of Plant Biology, University of La Coruña, E-15071, La Coruña, Spain
³IBMC, University of Porto, 4150 Porto, Portugal
*Author for correspondence Tel: +34 868 884945; Fax: +34 868 883963; E-mail: rosbarce@um.es

Abstract

Abstract:

Hypocotyl formation during the epigeal germination of seedlings is under strict hormonal regulation. In a 3 d old Zinnia elegans seedling system, gibberellic acid (GA₃) exerts an opposite effect to that exerted by light on hypocotyl photomorphogenesis because GA₃promotes an etiolated-like growth with an inhibition of radial (secondary) growth. For this reason, the effect of GA₃ on the basic peroxidase isoenzyme from Z. elegans (ZePrx), an enzyme involved in hypocotyl lignin biosynthesis, was studied. The results showed that GA₃ reduces ZePrx activity, similarly to the way in which it reduces seedling secondary growth. This hormonal response is supported by the analysis of the ZePrx promoter, which contains four types of GA₃-responsive cis-elements: the W Box/O2S; the Pyr Box; the GARE; and the Amy Box. Taken together, these results suggest that ZePrx is directly regulated by GA3, with this effect matching the inhibitory effect of GA on the hypocotyl secondary growth.

López Núñez-Flores MJ, Gutiérrez J, Gómez-Ros LV, Novo Uzal E, Sottomayor M, Ros Barceló A (2010) Downregulation of the basic peroxidase isoenzyme from Zinnia elegans by gibberellic acid. J. Integr. Plant Biol. 52(2), 244–251.

Key words: gibberellins, peroxidase, secondary growth, Zinnia elegans.

María Josefa López Núñez-Flores, Jorge Gutiérrez , Laura V. Gómez-Ros , Esther Novo Uzal, Mariana Sottomayor, and Alfonso Ros Barceló. Downregulation of the Basic Peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid[J]. J Integr Plant Biol., 2010, 52(2): 244-251.

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Downregulation of the Basic Peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid

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