J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (12): 1080-1085.DOI: 10.1111/j.1744-7909.2009.00880.x

• Metabolism and Biochemistry • Previous Articles     Next Articles

Determination of Essential Fatty Acid Composition among Mutant Lines of Canola (Brassica napus), through High Pressure Liquid Chromatography

Ghulam Raza1*, Aquil Siddique1, Imtiaz Ahmad Khan1, Muhammed Yasin Ashraf2 and Abdullah Khatri1   

  1. 1Plant Genetic Division, Nuclear Institute of Agriculture (NIA), Tando Jam, 70060, Pakistan
    2Nuclear Institute for Agriculture and Biology, Faisalabad, 38000, Pakistan
  • Received:2009-03-05 Accepted:2009-08-31 Published:2009-11-26
  • About author: *Author for correspondence Tel. +92 34 5676 6959; Fax: +92 2 2276 5284; E-mail: lalian77@yahoo.com


The present study aimed to quantify the methyl esters of lenoleic acid (LA), γ-lenolenic acid (LNA) and oleic acid (OL) in the oil of Brassica napus mutants. Five stable mutants (ROO-75/1, ROO-100/6, ROO-125/12, ROO-125/14, and ROO-125/17) of B. napus cv. ‘Rainbow’ (P) and three mutants (W97-95/16, W97-0.75/11 and W97-.075/13) of B. napus cv. ‘Westar’ (P) at M6 stage, exhibiting better yield and yield components, were analyzed for essential fatty acids. The highest seed yield was observed in the mutant (ROO-100/6) followed by ROO-125/14 of Rainbow, that is, 34% and 32% higher than their parent plants, respectively. Westar mutant W97-75/11 also showed 30% higher seed yield than its parent plant. High performance liquid chromatography analysis of the composition of fatty acids indicated that OL was the most dominant fatty acid, ranging from 39.1 to 66.3%; LA was second (15.3–41.6%) and LNA was third (18.1–28.9%). Mutant ROO-125/14 showed higher OL contents than parent (Rainbow). These results are expected to support the approval of ROO-125/14 in the National Uniform Varietal Yield Trials (NUVYT) as a new variety based on high oil quality.

Raza G, Siddique A, Khan IA, Ashraf MY, Khatri A (2009). Determination of essential fatty acid composition among mutant lines of canola (Brassica napus L.), through high pressure liquid chromatography. J. Integr. Plant Biol. 51(12), 1080–1085.

Key words: Brassica, HPLC, lenoleic acid, lenolenic acid, oleic acid.

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