J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (4): 360-366.DOI: 10.1111/j.1744-7909.2008.00803.x

• Cell and Developmental Biology • Previous Articles     Next Articles

Quantitative Trait Loci Mapping of Flag-leaf Ligule Length in Rice and Alignment with ZmLG1 Gene

Dali Zeng1, Jiang Hu1, Guojun Dong1, Jian Liu1, Longjun Zeng1, Guangheng Zhang1, Longbiao Guo1, Yihua Zhou2 and Qian Qian1*   

  1. 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
    2Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China
  • Received:2008-08-06 Accepted:2008-11-06 Published:2009-02-05
  • About author:*Author for correspondence E-mail: qianqian188@hotmail.com
  • Supported by:
    Supported by the State Key Basic Research and Development Plan of China (2007CB10920203), the National Natural Science Foundation of China (30771160, 30571136), the Open Fund of State Key Laboratory of Rice Biology (080104), and the Research Program of Zhejiang Province.


A doubled haploid (DH) population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid ‘CJ06’/‘TN1’, was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci (QTL) mapping using the DH population. Five QTLs (qLL-2, qLL-4, qLL-6, qLL-10 and qLL-12) controlling the ligule length (LL) were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL-4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.

Zeng D, Hu J, Dong G, Liu J, Zeng L, Zhang G, Guo L, Zhou Y, Qian Q (2009) Quantitative trait loci mapping of flag-leaf ligule length in rice and alignment with ZmLG1 gene. J. Integr. Plant Biol. 51(4), 360–366.

Key words: double haploid population, in silico mapping, ligule length of flag leaf, quantitative trait loci, rice.

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