J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (11): 982-992.DOI: 10.1111/j.1744-7909.2009.00870.x

Special Issue: Rice Genomics and Agriculture

• Cell and Developmental Biology •     Next Articles

Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice

Shaohong Qu1,2*, Jong-Seong Jeon3,4, Pieter B.F. Ouwerkerk5, Maria Bellizzi1, Jan Leach6, Pamela Ronald3 and Guo-Liang Wang1,7*   

  1. 1 Department of Plant Pathology, Ohio State University, Columbus OH 43210, USA
    2 Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    3 Department of Plant Pathology, University of California, Davis CA 95616, USA
    4 Graduate School of Biotechnology and Plant Metabolism Research Center, Yongin 446-701, Korea
    5 Institute of Biology, Leiden University, Clusius Laboratory, RA Leiden 2300, The Netherlands
    6 Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USA
    7 Crop Gene Engineering Key Laboratory of Hunan Province and Pre-State Key Laboratory of Crop Germplasm Renovation and Resource Utilization, Hunan Agricultural University, Changsha 410128, China
  • Received:2009-06-18 Accepted:2009-07-27 Published:2009-11-10
  • About author: * *Authors for correspondence
    Tel: +86 571 8641 9018; Fax: +86 571 8640 4256; E-mail: squ111@163.com or Tel: +1 614 292 9280; Fax: +1 614 292 4455; E-mail: wang.620@osu.edu

Abstract:

Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches: (i) AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain (GVG) chemical-inducible expression system; (ii) deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and (iii) suppression of early transposition  rice callus through a dual-functional hygromycin resistance gene in a novel Ds element (HPT-Ds). We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community.
 

Qu S, Jeon JS, Ouwerkerk PBF, Bellizzi M, Leach J, Ronald P, Wang GL (2009). Construction and application of efficient Ac-Ds transposon tagging vectors in rice. J. Integr. Plant Biol. 51(11), 982–992.

Key words: Ac-Ds transposable element, GVG-inducible expression, Cre-lox site-specific recombination, rice.

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