Effects of Crosslinking Agent-DFDNB on Energy-transducing Reactions of Chloroplast Thylakoid Membrane Proteins

Abstract

Abstract: The effects of crosslinking agent-DFDNB (difluoro dinitro benzene) on functions of chloroplast thylakoid membrane proteins were investigated. DFDNB inhibited activities of PSP and membrane-bound ATPase in chloroplasts. It decreased proton uptake of light-inducted chloroplast thylakoids and the relative value of fluorescence quenching of 9-aminoacridine, and inhibited the rate of fast electrogenic phase of absorption change at 515 nm in chloroplasts. In addition, the isolated CF1-ATPase was crosslinked with DFDNB. The pattern of polymers of crosslinked CF1-ATPase was observed on SDS-PAGE.

Key words: DFDNB (difluore dinitro benzene), Thylakoids, Protein, Energy-trasduc-ing reaction, Crosslinking

Wang Guo-qiang,Zhang Yin-ping,Qian Yue-qin and Peng Jian-xin. Effects of Crosslinking Agent-DFDNB on Energy-transducing Reactions of Chloroplast Thylakoid Membrane Proteins[J]. J Integr Plant Biol., 1993, 35(11): -.

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Effects of Crosslinking Agent-DFDNB on Energy-transducing Reactions of Chloroplast Thylakoid Membrane Proteins

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