Abstract: Gastrodia data B1. antifungal protein (GAFP) was purified by DEAE-Cellulase column chromatography and Sephadex G-50 column chromatography, and the polyclonal antibody against GAFP (GAFP-PcAbs) was also produced. Using Western blot and Dot blot it was demonstrated that GAFP-PcAbs is specific for GAFP. For further study, immunofluorescence localization of GAFP in secondry corms of G. data has been conducted with fluorescence microscopy. The result showed that GAFP was localized at epidermal and cortical tissues in secondary corm of G. elata,and the content of GAFP was higher in epidermis.

Key words: Gastrodia elata, Antifungal protein, Serological antigen, Western blot, Dot blot, Immunofluorescence