J Integr Plant Biol ›› 2009, Vol. 51 ›› Issue (6): 614-621.DOI: 10.1111/j.1744-7909.2008.00801.x

• New Technology • Previous Articles    

Development of a Low-cost Polymerase Chain Reaction-based Method for Studying Differentially Expressed Genes in Developing Rice Leaves

Yin-Wan Wendy Fung1 , Hoi Yee Chow2, Tik Wan Law1, Biao Dong2 and Hoi Shan Kwan2*   

  1. 1 Faculty of Science, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China
    2 Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China
  • Received:2008-07-04 Accepted:2008-10-20 Published:2009-01-31
  • About author: *Author for correspondence Tel: +852 2609 6110; Fax: +852 2603 5646; E-mail: hoishankwan@cuhk.edu.hk

Abstract:

Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction (RAP-PCR) and dot blot hybridization methods to screen a rice leaf primordium cDNA library. Three developmental stages during vegetative growth were examined. The cDNA clones showing different hybridization patterns were further analyzed and verified. Here we demonstrate that the combination of RAP-PCR and dot blot hybridization could provide an efficient and relatively low-cost cDNA library screening approach to discover genes not previously known to be associated with leaf development in rice. We believe that the findings described here will help to elucidate the molecular mechanism(s) underlying the developmental processes of rice leaf.

Fung YWW, Chow HY, Law TW, Dong B, Kwan HS (2009). Development of a low-cost polymerase chain reaction-based method for studying differentially expressed genes in developing rice leaves. J. Integr. Plant Biol. 51(6), 614-621.

Key words: cDNA clones, development, dot blot hybridization, primordium, RNA arbitrarily primed-polymerase chain reaction.

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