J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (9): -.

• Research Articles •    

Protoplast Culture and Plant Regeneration of Winter Wheat (Triticum aestivum L.)

Li Hong-chao, Yang Xiao-hui, Hu Dao-fen, Liu Jian-ping and Guo Zhong-chen   

Abstract: Suspension cultures were established from embryogenic calli derived from cultured anthers of cv. Jinghua No.1 and mature embryos of cv. Youmangbai No. 7, respectively. After being isolated and cultured in WPMI, protoplasts began to form cell walls within 1 day post-isolation, followed by cell division observed between 2–3 days. A division frequency of 22.0% was estimated on the 7th day of culture, and 43.7% on the 14th day. During 10–15 days after the initiation of culture, a large number of cell aggregates emerged, with 0.5–0.8% of plating efficiency. Protoplast-derived calli grew up to lmm or more in diameter when cultured for 4 weeks, and eventually gave rise to green plants through embryogenesis and organogenesis after being transferred to differentiation media. Plant regeneration from protoplasts was already obtained from Jinghua No.l, and protoplast-derived calli from Youmangbai No.7; an experiment on organ differentiation for the latter is under way. A few factors affecting the protoplast cultures were also studied.

Key words: Winter wheat, Protoplast culture, Plant regeneration

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