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J Integr Plant Biol, 1995, 37 (11): -, Research Article
Enhancement of Plant Regeneration Rate of Brassica parachinensis Cultured in Vitro
Zhang Peng and Ling Ding-hou
doi:
Abstract
Among the major factors affected plant regeneration in Brasica parachinensis, a combination of BAP (bacterial alhaline phosphatase) and NAA, at a concentration of 2 mg/L and 1 mg/L respectively, could raise 26.8% of the regeneration rate. It was also found that Ag- NO3 or ABA when supplemented in the culture medium could increase the plant regeneration rate by 79. 0% and 32.30% respectively, indicating that AgNO3 was superior to ABA as a single factor. With a combination of AgNO3 (4 mg/L) and ABA (0. 5 mg/L) added to MS medium already supplemented with ABA 2.0 mg/L and NAA 1.0 mg/L the authors had achieved remarkable results in frequency increase of 89.0%, 84.3% and 86.0% in three explant varieties of B. parachinensis, viz "49-19', "60D' and "70D', respectively. Among the three explants (cotyledon, hypocotyl and petiole with cotyledon), petiole with cotyledon possessed the highest capability for plant regeneration. In addition the age of seedlings and mode of inoculations also influenced the frequency of plant regeneration.' Histological observation evidenced that the mode of plant regeneration in B. parachinensis was of organogenesis. Adventitions buds derived from the cells of vascular parenchyma at the cut surface of petioles. Mature plants were developed after the plantlets were transferred into the plot.
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