J Integr Plant Biol. ›› 2012, Vol. 54 ›› Issue (3): 169-179.DOI: 10.1111/j.1744-7909.2012.01100.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

ROP11 GTPase is a Negative Regulator of Multiple ABA Responses in Arabidopsis

Zixing Li, Jun Kang, Ning Sui and Dong Liu*   

  1. MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China
  • Received:2011-12-20 Accepted:2012-01-05 Published:2012-01-10
  • About author:*Corresponding author. Tel: +86 10 6278 3603; Fax: +86 10 6278 3603; E-mail: liu-d@tsinghua.edu.cn

Abstract:

The phytohormone abscisic acid (ABA) plays crucial roles in plant development and plant responses to environmental stresses. Although ABA receptors and a minimal set of core molecular components have recently been discovered, understanding of the ABA signaling pathway is still far from complete. In this work, we characterized the function of ROP11, a member of the plant-specific ROP small GTPases family, in the ABA signaling process. ROP11 is preferentially expressed in guard cells in all plant organs with stomata. Expression of a constitutively active ROP11 (CA-ROP11) suppresses ABA-mediated responses, whereas reduced expression of ROP11 or expression of its dominant-negative form (DN-ROP11) causes the opposite phenotypes. The affected ABA-mediated responses by ROP11 include seed germination, seedling growth, stomatal closure, induction of ABA-responsive genes, as well as plant response to drought stress. Furthermore, we showed that ROP11 and its closest-related family member, ROP10, act in parallel in mediating these responses. ABA treatment does not affect ROP11 transcription and protein abundance; however, it causes the accumulation of CA-ROP11 in the nucleus. These results demonstrated that ROP11 is a negative regulator of multiple ABA responses in Arabidopsis.

Li Z, Kang J, Sui N, Liu D (2012) ROP11 GTPase is a negative regulator of multiple ABA responses in Arabidopsis. J. Integr. Plant Biol. 54(3),169–179.

Key words: ROP11 GTPase, ABA responses, negative regulator, rop10rop1, double mutant, Arabidopsis

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