J Integr Plant Biol. ›› 2012, Vol. 54 ›› Issue (3): 152-168.DOI: 10.1111/j.1744-7909.2012.01103.x

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

Glyoxylate Reductase Isoform 1 is Localized in the Cytosol and Not Peroxisomes in Plant Cells

Steven L. K. Ching1, Satinder K. Gidda1, Amanda Rochon2, Owen R. van Cauwenberghe2, Barry J. Shelp2 and Robert T. Mullen1*   

  1. 1Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    2Department of Plant Agriculture, University of Guelph, Guelph, Ontario N1G 2W1, Canada
  • Received:2011-11-15 Accepted:2012-01-26 Published:2012-02-06
  • About author:*Corresponding author. Tel: +519 824 4120 x56479; Fax: + 519 837 1802; E-mail: rtmullen@uoguelph.ca

Abstract:

Glyoxylate reductase (GLYR) is a key enzyme in plant metabolism which catalyzes the detoxification of both photorespiratory glyoxylate and succinic semialdehdye, an intermediate of the γ-aminobutyrate (GABA) pathway. Two isoforms of GLYR exist in plants, GLYR1 and GLYR2, and while GLYR2 is known to be localized in plastids, GLYR1 has been reported to be localized in either peroxisomes or the cytosol. Here, we reappraised the intracellular localization of GLYR1 in Arabidopsis thaliana L. Heynh (ecotype Lansberg erecta) using both transiently-transformed suspension cells and stably-transformed plants, in combination with fluorescence microscopy. The results indicate that GLYR1 is localized exclusively to the cytosol regardless of the species, tissue and/or cell type, or exposure of plants to environmental stresses that would increase flux through the GABA pathway. Moreover, the C-terminal tripeptide sequence of GLYR1, -SRE, despite its resemblance to a type 1 peroxisomal targeting signal, is not sufficient for targeting to peroxisomes. Collectively, these results define the cytosol as the intracellular location of GLYR1 and provide not only important insight to the metabolic roles of GLYR1 and the compartmentation of the GABA and photorespiratory pathways in plant cells, but also serve as a useful reference for future studies of proteins proposed to be localized to peroxisomes and/or the cytosol.

Ching SLK, Gidda SK, Rochon A, van Cauwenberghe OR, Shelp BJ, Mullen RT (2012) Glyoxylate reductase isoform 1 is localized in the cytosol and not peroxisomes in plant cells. J. Integr. Plant Biol. 54(3), 152–168.

Key words: Arabidopsis, GABA, GLYR1, cytosol, photobleaching, peroxisome, PTS stress tobacco BY-2

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