Cloning and Sequencing of phbB and phbC in Alcaligenes eutrophus H16 and Construction of Their Plant Expression Vectors

Abstract

Abstract: NADPH-dependent acetoacetyl-CoA reductase gene (phbB) and poly-β-hydroxybu-tyrate (PHB) synthase gene (phbC) for biosynthesis of PHB were amplified and cloned from chromosomal DNA of Alcaligenes eutrophus H16 using PCR. The restriction maps and sequencing results show that both phbB and phbC have been cloned. It was found that the two genes cloned were highly homologous in DNA sequences to those being reported abroad. By DNA processing, the authors have constructed three tuber-specific plant expression vectors: pPSAGB (containing phbB), pBIBGC (containing phbC) and pPSAGCB (containing both phbB and phbC).

Key words: Polymerase chain reaction, phbB, phbC, Gene cloning, Plant expression vectors

Xie An-yong, Cui Xiao-jiang and Song Yan-ru. Cloning and Sequencing of phbB and phbC in Alcaligenes eutrophus H16 and Construction of Their Plant Expression Vectors[J]. J Integr Plant Biol., 1995, 37(8): -.

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Cloning and Sequencing of phbB and phbC in Alcaligenes eutrophus H16 and Construction of Their Plant Expression Vectors

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