Cloning and Sequencing of phbB and phbC in Alcaligenes eutrophus H16 and Construction of Their Plant Expression Vectors

Abstract

Abstract: NADPH-dependent acetoacetyl-CoA reductase gene (phbB) and poly-β-hydroxybu-tyrate (PHB) synthase gene (phbC) for biosynthesis of PHB were amplified and cloned from chromosomal DNA of Alcaligenes eutrophus H16 using PCR. The restriction maps and sequencing results show that both phbB and phbC have been cloned. It was found that the two genes cloned were highly homologous in DNA sequences to those being reported abroad. By DNA processing, the authors have constructed three tuber-specific plant expression vectors: pPSAGB (containing phbB), pBIBGC (containing phbC) and pPSAGCB (containing both phbB and phbC).

Key words: Polymerase chain reaction, phbB, phbC, Gene cloning, Plant expression vectors

Xie An-yong, Cui Xiao-jiang and Song Yan-ru. Cloning and Sequencing of phbB and phbC in Alcaligenes eutrophus H16 and Construction of Their Plant Expression Vectors[J]. J Integr Plant Biol., 1995, 37(8): -.

[1]	Zhirui Yang and Feng Qin. The battle of crops against drought: Genetic dissection and improvement [J]. J Integr Plant Biol., 2023, 65(2): 496-525.
[2]	Jingyang Gao, Peijiang Feng, Jingli Zhang, Chaopei Dong, Zhao Wang, Mingxiang Chen, Zhongliang Yu, Bowen Zhao, Xin Hou, Huijuan Wang, Zhaokun Wu, Razia Sultana Jemim, Haidong Yu, Doudou Sun, Pei Jing, Jiafa Chen, Weibin Song, Xuecai Zhang, Zijian Zhou and Jianyu Wu, . Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion [J]. J Integr Plant Biol., 2023, 65(12): 2645-2659.
[3]	Sujay Paul, Anirban Kundu, and Amita Pal. Identification and expression profiling of Vigna mungo microRNAs from leaf small RNA transcriptome by deep sequencing [J]. J Integr Plant Biol., 2014, 56(1): 15-23.
[4]	Dongli He, Chao Han, and Pingfang Yang. Gene Expression Profile Changes in Germinating Rice [J]. J Integr Plant Biol., 2011, 53(10): 835-844.
[5]	Yin-Wan Wendy Fung, , Hoi Yee Chow, Tik Wan Law, Biao Dong, and Hoi Shan Kwan. Development of a Low-cost Polymerase Chain Reaction-based Method for Studying Differentially Expressed Genes in Developing Rice Leaves [J]. J Integr Plant Biol., 2009, 51(6): 614-621.
[6]	Yan Hu, Wang-Zhen Guo and Tian-Zhen Zhang. Construction of a Bacterial Artificial Chromosome Library of TM-1, a Standard Line for Genetics and Genomics in Upland Cotton [J]. J Integr Plant Biol., 2009, 51(1): 107-112.
[7]	Wei Gong, Zhen Zeng, Ye-Ye Chen, Chuan Chen, Ying-Xiong Qiu and Cheng-Xin Fu. Glacial Refugia of Ginkgo biloba and Human Impact on Its Genetic Diversity: Evidence from Chloroplast DNA [J]. J Integr Plant Biol., 2008, 50(3): 368-374.
[8]	Ping-Hua Chen, Yong-Bao Pan and Ru-Kai Chen. High-throughput Procedure for Single Pollen Grain Collection and Polymerase Chain Reaction in Plants [J]. J Integr Plant Biol., 2008, 50(3): 375-383.
[9]	Mei-Qin Liu, Xin Shen, Wei-Lun Yin and Cun-Fu Lu. Changes of 5'' Terminal Nucleotides of PCR PrimersCausing Variable T-A Cloning Efficiency [J]. J Integr Plant Biol., 2007, 49(3): -.
[10]	An-Ding LUO, Luo LIU, Zuo-Shun TANG, Xian-Quan BAI, Shou-Yun CAO and Cheng-Cai CHU. Down-Regulation of OsGRF1 Gene in Rice rhd1 Mutant Results in Reduced Heading Date [J]. J Integr Plant Biol., 2005, 47(6): 745-752.
[11]	XU Hong, ZHANG Xiang-Qi, WANG Xian-Ping, GUO Ai-Guang. Molecular Cloning of Agropyron intermedium Low-Molecular-Weight Glutenin Subunit Genes from a Triticum aestivum-Ag. intermedium Addition Line TAI-13 [J]. J Integr Plant Biol., 2004, 46(5): 595-602.
[12]	QIN Qiao-Ping, ZHANG Shang-Long, CHEN Jun-Wei, XIE Ming JIN Yong-Feng, CHEN Kun-Song, Syed ASGHAR. Isolation and Expression Analysis of Fructokinase Genes from Citrus [J]. J Integr Plant Biol., 2004, 46(12): 1408-1415.
[13]	XIA Ming, WANG Xiao Bing, LI Hai Bo, WU Ping. Identification of the Rice Vacuolar ATPase B Subunit Gene and Its Expression Pattern Analysis Under Phosphorus Deficiency [J]. J Integr Plant Biol., 2002, 44(5): 573-578.
[14]	ZHAO Da-Zhong, CHEN Dan-Ying, CHEN Min, LIU Chun-Xiang, YANG Cheng and ZHAI Zhong-He. The Existence of Intermediate Filament in Arabidopsis thaliana Cell [J]. J Integr Plant Biol., 1999, 41(8): -.
[15]	PENG Kai-Man and ZHANG Qi-Fa. Acosmid Library Constructed to the Elite Rice Cultivar [J]. J Integr Plant Biol., 1999, 41(3): -.

Cloning and Sequencing of phbB and phbC in Alcaligenes eutrophus H16 and Construction of Their Plant Expression Vectors

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments