J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (6): -.

• Research Articles •    

PCR Amplification and Sequencing of a Wheat rbc S Gene Promoter

Zeng Wei-ke, Wang Jin-zhao, Feng Yu, Ding Yue-yun, Wu Nai-hu and Ma Cheng   

Abstract: The promoter region of the rbc S gene from Triticum aestivum L. FAN-61 was amplified by PCR. Two commonly used restriction enzyme sites, Barn HI and Sma Ⅰ, were respectively added to the 5′ and 3′ ends of the promoter fragment in order to make it easier for subsequent manipulation. The amplified fragment was cloned into pUC18/19 vectors and its sequence was determined. The 704 bp promoter fragment contained a TATA Box (TATATATA) and an Ⅰ Box (GATAAT). The sequence of CCAAC in it was similar to the sequence of CCAAT found upstream of other eukaryotic genes. Moreover, it also contained the consensus sequences of CCACA and GAACGTGAGCCA which were present in those of other monocot rbc S.

Key words: Wheat, rbc S promoter, Polymerase chain reaction, Sequencing of nu-Cleotide

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q