J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (11): -.

• Research Articles •    

The Role of Cytokinin Biosynthetic Gene in Regulating the Expression of a Class of Pathogenesis-Related Protein Genes in Tobacco Plants

Ma Qing-hu, Song Yan-ru and Sun Jing-san   

Abstract: The expression characteristics of a class of pathogenesis-related protein (PR) genes, namely basic chitinase, β-1, 3-glucanase, osmotin and extensin, were studied in tobacco (Nicotiana tabacum cv. Wisconsin 38) plants. RNA blot hybridization showed that these four genes were regulated in a developmental and organ-specific manner in tobacco. In the transgenic fascicular shoots which contained the active cytokinin biosynthetic gene (ipt gene) from Agrobacterium tumefaciens, the expressions of these four genes were co-regulated by overproduction of endogenous cytokinins and vector effect. Cytokinins reduced the expressions while vector effect induced the expressions of these four genes. Heat shock also de creased the steady-state levels of the four RNAs. These data suggest a complex regulation of PR genes.

Key words: Cytokinin biosynthetic gene, Vector effect, Pathogenesis-related protein genes

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q