J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (7): -.

• Research Articles •    

Ethylene Induction of Apoptosis in Carrot Protoplasts

ZHOU Jun, ZHU Hai-Zhen, JIANG Xiao-Fang and DAI Yao-Ren   

Abstract: Ethylene is an important plant hormone involving in various physiological processes. Ethrel which releases ethylene in a solution of pH higher than 4.1, was used to treat the carrot protoplasts. It was found that percentage of cell death kept rising with the increase of ethrel concentration. Characteristics of apoptotic cells, such as nuclear condensation and DNA laddering were observed. Neutral comet assay which detects nuclear DNA fragmentation provided evidence that ethylene induced apoptosis in carrot protoplasts.

Key words: Apoptosis, Ethylene, Daucus carota, Comet assay, DNA laddering

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