Abstract: Genus Pseudotaxus distributed in the Zhejiang, Hunan, Jiangxi etc. is endemic to China. Only one species is envolved in the genus. A preliminary investigation on chemical components of Pseudotaxus is reported in the present paper. Three crystalline substances have been isolated from trunk of P. chienii grown in Zhejiang and have been identified as llβ, 22-dihydroxyhopane (Ⅰ), β-sitosterol (Ⅱ) and tsugalacton (Ⅲ) respectively by chemical and spectral (IR, NMR, MS) analyses.