J Integr Plant Biol. ›› 1981, Vol. 23 ›› Issue (5): -.

• Research Articles •    

Gyto-Histological Studies of Plantlets Derived from the Explants of Chrysanthemum Receptacles

Guan He, Li Ren-guei, and Cao Han-min   

Abstract: In the MS basic media supplemented with either 6-Benzyl amino purine (6-BA) or 2, 4-dichlorophenoxyacetie acid (2, 4-D), plantlets were induced from receptacle explants of chrysanthemum. Shoots were grown at first in this media with 6-BA 2-16 mg/l. Then they were transplanted into MS media supplemented with IAA 1 mg/l to root. Eventually they formed plantlets. The receptacle explants induced with 2, 4-D 2mg/l were surrounded by numerous roots. Finally plantlets were formed. Tubular flowers with colours of original rarity were grown out from some receptacle explants in media supplemented with 6-BA 8 mg/l. Under the influence of 6-BA, some parenchymatous cells changed into embryonic cells with characteristic of large nucleus, rich protoplasm and capable of dividing. These embryonic cells developed into meristematic nodules and vascular tissue nodules step by step and were differentiated into bud primodia at the surface of the receptacle explants. There is vascular connection between vascular tissue nodule and bud. The bud primordia developed into shoot. Many proplastids were formed in the protoplasm of the induced receptacle explant cells and proplastids developed into chloroplasts gradually.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22