J Integr Plant Biol. ›› 1981, Vol. 23 ›› Issue (1): -.

• Research Articles •    

Chromosome Banding Technique with Trypsin-Giemsa in Plants

Zhang Zi-li and Chen Gui-lan   

Abstract: The present paper is the investigation for the chromosome banding technique of trypsin-Giemsa in plants. Vicia faba, Allium cepa and Secale cereale were used. The fixed roots were hydrolysed in 0.1 N HCl or 45% acetic acid and squashed in a drop of 45% acetic acid. Coverslips were removed by the freezing method and the slides dried in the air. The preparations were then immersed in 0.025% solution of trypsin in phosphate-buffered saline (pH 7.2) at 34 ℃ for 10–15 minutes. Staining was performed on 10% Giemsa at pH 7.2. This method has some unique features. It is fast and technically simple and C-banding of good-quality can be consistently demonstrated. In this paper the chromosome banding pattern with trypsin-Giemsa in Vicia faba is analyzed and the mechanism of chromosome banding with trypsin-Giemsa is also discussed.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q