J Integr Plant Biol. ›› 1979, Vol. 21 ›› Issue (1): -.

• Research Articles •    

Plantlets from Isolated Pollen Cultures of Eggplant (Solanum melongena L.)

Gu Shu-rong   

Abstract: The present paper reports the experimental results on isolated pollen cultures of eggplant. The pretreatment of culturiug pollen grains within the anthers for four days before isolation was beneficial to the division of pollen grains and formation of calluses. The best liquid medium tested for isolated pollen cultures was composed of MS maeroelements and supplemented with 800 mg/l glutamine, 100 mg/l serine, 5 g/l myoinositol, 2 mg/l 1, 4-D and 1 mg/l kinetin. The formation of pollen calluses was mostly derived from the division of the vegetative nucleus, and a part of calluses was developed from the equal division of the microspores. After the isolated pollen grown in suspensions for about 20 days under static culture the fresh medium was added and at the same time the cultures were kept under rotating condition. These measures were beneficial to the production and development of calluses. When the calluses grew up to 3–4 mm. in diameter, they were transferred to the semisolid medium in which MS medium was used as the basic one for differentiation of the organs. Plantlets were finally formed from the pollen calluses derived from the cultivar, "the nine-leaved eggplant.

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