Abstract: The present paper deals with the comprehensive utilization of the medicinal plant resource of Berberis poiretii based on their botanical, phytochemieal, pharmacological and clinical aspects. A major alkaloid was isolated from the waste residue of berberine isolation and identified by IR, NMR and MS as berbamine, which was demonstrated to be an effective agent to increase leucocyte in the treatment of 167 leukopenia patients. The result of qualitative and quantitative analysis of 22 species of medicinal Berberis was also provided.