J Integr Plant Biol. ›› 1978, Vol. 20 ›› Issue (3): -.

• Research Articles •    

Anther Culture and Androgenesis of Rye

Sun Jing-san, Zhu Zhi-qing and Wang Jing-ju   

Abstract: The nucleus vegetative in nature derived from unequal or equal division of the uninucleate pollen in rye anthers cultured in vitro develops into pollen embryoids or calli. The green haploid plantlets are differentiated successfully. The frequency of callus and embryoid formation may be efficiently increased when the spikes are pretreated at 1–3 ℃ for 7 days before inoculation. According to the condition in which the multinucleate (free nuclei) and multi-cellular pollen grains become abortive, it is suggested that the formation of new walls between the dividing cells and the opportune rupture of the pollen exine are the two critical factors, which determine whether the embryoids and calli will be able to develop once the microspores are initiated.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q