J Integr Plant Biol. ›› 1975, Vol. 17 ›› Issue (2): -.

• Research Articles •    

Artificial Culture of Embryo in Citrus

Wang Ta-yung and Chang Chin-jen   

Abstract: In an attempt to promote the efficiency in citrus breeding and estimate the possibility of embryo culture applied to citrus production, we carried out the culture technique in the hybrid embryos (Citrus sinensis Osbeek × Poncirus trifoliata Raf.). The results are summarized as follows: The fifth small embryos of the hybrid developed into seedings on White's medium containing IAA (l ppm) and CH (600 ppm) and on the MT medium containing GA (1 ppm), but some of these embryos cultured on the MT medium containing 2, 4-D (0.7 ppm) and BA (0.23 ppm) produced callus at first, and formed roots and plantlets from the original embryos later on. When the callus tissues excised from the embryos were transferred into the MT medium supplemented with BA (5 ppm) and IAA (1 ppm), they produced buds and developed into shoots, but no root was formed. When they were again transferred into a medium containing NAA (1 ppm) only, they could form roots and they developed into perfect plantlets as well. According to these studies it seems practical that the embryo culture technique could be used to study the distribution of zygote embryos and to overcome the interference of nucellus embryos in citrus breeding.

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