Abstract: The present article deals with the morphological and the anatomical observations on the mature sporophores in Ganoderma lucidum. The developmental morphology of the exochite and the basidiospores have been investigated, inter alia, in some detail. After investigating the effects of CO2 gas on the sporophore culture the authors consider that when CO2 content in the atmosphere is increased to 0.1% it promotes the growth of the stipe and inhibits the growth and differentiation of the pileus, and this is the main cause of the formation of the deformed sporophores. At the same time it has been observed that the deformed sporophores which did not give rise to pileus could produce pileus if CO2 content in the atmosphere decreased. It is suggested that in the early stage of the sporophore culture, the CO2 content in the atmosphere should be increased adequately in order to promote the growth of the stipe, in the later stage CO2 content should be decreased by ventilation to induce the differentiation and growth of the pileus; thus disadvantageous factors may be converted into advantageous ones. The basidiospore germination has been obtained by the liquid culture with 10%–20% sporophore extract.The germination percentage is about 8%–15%. Thus, a new method for spawn rejuvenation is found.