J Integr Plant Biol. ›› 1958, Vol. 7 ›› Issue (3): -.
• Research Articles •
Tang Su
Abstract: The catechins of fresh leaves of Lung-Chin tea had been paper chromatographied on Whatman No.1 filter paper by using phenol-water, n-butanol-acetic-acid-water as solvents. In several batches, the juice was autoclaved under 15 1bs. pressure for 15 minutes, or inoculated with Aspergillus Niger. The results were shown in Fig, 1 and 2. Spots 1, 2, 6, 8, 10, 4, B, and Y were always present, while 9, 7, 3, and G appeared less frequently. The spots 1, 2, 6, 8, 10, 4, 9, 7, and 3 had been tentatively identified as m-digallic acid, 1-epigallocatechin, 1-epigallocatechin gallate, 1-epicatechin gallate, 1-epicatechin, gallic acid, dl-catechin, catechin α-gallate and dl-gallocatechin respectively. Spots B, G, and Y had never been mentioned by other workers. Spot B is probably a gallocatechin or a gallate of other polyphenol as it turns blue on spraying with FeCl3 solution and is not hydrolyzed by Aspergillus Niger. Spot Y is probably an organic acid as it is shown by bromocresol green. Spot G has not yet identified, it is richer in stems than in leaves.
Tang Su. A Preliminary Report on the Paper Chromatography of the Catechins of Lung-Chin Tea Leaves[J]. J Integr Plant Biol., 1958, 7(3): -.
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