Author: Tang Su
J Integr Plant Biol 1958, 7 (3): -.
The catechins of fresh leaves of Lung-Chin tea had been paper chromatographied on Whatman No.1 filter
paper by using phenol-water, n-butanol-acetic-acid-water as solvents. In several batches, the juice was
autoclaved under 15 1bs. pressure for 15 minutes, or inoculated with Aspergillus Niger. The results were
shown in Fig, 1 and 2. Spots 1, 2, 6, 8, 10, 4, B, and Y were always present, while 9, 7, 3, and G appeared
less frequently. The spots 1, 2, 6, 8, 10, 4, 9, 7, and 3 had been tentatively identified as m-digallic acid,
1-epigallocatechin, 1-epigallocatechin gallate, 1-epicatechin gallate, 1-epicatechin, gallic acid, dl-catechin,
catechin α-gallate and dl-gallocatechin respectively. Spots B, G, and Y had never been mentioned by
other workers. Spot B is probably a gallocatechin or a gallate of other polyphenol as it turns blue on spraying
with FeCl3 solution and is not hydrolyzed by Aspergillus Niger. Spot Y is probably an organic acid as it is
shown by bromocresol green. Spot G has not yet identified, it is richer in stems than in leaves.