J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (10): 1243-1249.

• Research Articles • Previous Articles     Next Articles

Enzymatic Degradation of Parvifloside

JIN Jian Ming, LIU Xi Kui, TENG Rong Wei, YANG Chong Ren*   

  • Published:2002-10-21


Parvifloside (1), a new furostanol pentaglycoside, was isolated from the fresh rhizomes of Dioscorea parviflora C. T. Ting. On the basis of spectroscopic and chemical methods, its structure was elucidated as (25R) 26 O β glucopyranosyl furost 5 en 3β,22ξ,26 triol 3 O βD glucopyranosyl (1→3) βD glucopyranosyl (1→4) [αL rhamnopyranosyl (1→2)] βD glucopyranoside. Six prosapogenins (2-7) were obtained from the enzymatic degradation of 1 by cellulase, but only 3 and 4 were obtained by β glucosidase. The structures of all compounds were determined by spectroscopic data. The activity of the isolated compounds on deformation of mycelia germinated from Pyricularia oaryzae P 2b conidia was evaluated.

金建明 刘锡葵 滕荣伟 杨崇仁*


摘要: 从小花盾叶薯蓣 (Dioscoreaparviflora C.T .Ting)的新鲜根状茎中分离到一个新的呋甾烷型配糖体 ,命名为小花盾叶薯蓣甙 (parvifloside) (1) ,其结构通过波谱和化学方法鉴定为 :(2 5R)-2 6-O-β-glucopyranosyl-furost-5-en-3β ,2 2 ξ ,2 6-triol 3-O-β-D-glucopyranosyl (1→ 3)-β-D-glucopyranosyl (1→ 4 )-[α-L-rhamnopyranosyl (1→ 2 ) ]-β-D-glucopyra noside。化合物 1在纤维素酶粗酶和 β_葡萄糖苷酶中进行水解 ,得到降解产物 2 - 7。对 1的酶解现象进行了讨论。同时 ,对所分离的甾体皂甙的抗稻瘟霉菌活性进行了初步筛选。

关键词: 小花盾叶薯蓣;小花盾叶薯蓣甙;甾体皂甙;酶降解

通讯作者。Tel.:+86-871-5223424;Fax: +86-871-5150124;E -mail: cryang @ public.km.yn. cn;glycoside @ maul.kib.ac.cn.

Key words: Dioscorea parviflora, parvifloside, steroidal glycoside, enzymatic degradation

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