Effect of Nitrogen Form on the Activity of Tonoplast Pyrophosphatase in Tomato Roots (in English)

Abstract

Abstract:

Effect of nitrate and ammonium on the activity of tonoplast pyrophosphatase (V-PPase) was investigated in the roots of tomato ( Lycopersicon esculentum L.). The results showed that the activity of V-PPase was increased by ammonium nutrition, compared with nitrate nutrition. The H+ transport of tonoplast vesicles by V-PPase was also stimulated by ammonium nutrition. The result of Western blot indicated that the protein amount of V-PPase was increased by ammonium nutrition.

氮素形态对番茄根系液泡膜焦磷酸酶活性的影响
朱祝军¹ 钱亚榕¹ Wolfgang PFEIFFER ²

（1.浙江大学农业与生物技术学院园艺系，杭州310029；
2. 奥地利萨尔茨堡大学植物生理研究所，奥地利萨尔茨堡5020）

摘要：采用超速梯度离心提取液泡膜微囊,研究硝态氮和铵态氮供应情况下番茄(Lycopersicon esculentum L.)根系液泡膜焦磷酸酶活性的变化.结果表明:供应铵态氮番茄根系专一性液泡膜焦磷酸酶活性比供应硝态氮的高37.7%;供应铵态氮根系液泡膜焦磷酸酶转运质子活力也明显高于供应硝态氮根系. Western blot结果表明,供应铵态氮根系液泡膜焦磷酸酶蛋白表达量也明显增加.

关键词：番茄；氮素形态；液泡膜；焦磷酸酶

Key words: tomato, nitrogen form, tonoplast, pyrophosphatase

ZHU Zhu-Jun, QIAN Ya-Rong and Wolfgang PFEIFFER. Effect of Nitrogen Form on the Activity of Tonoplast Pyrophosphatase in Tomato Roots (in English)[J]. J Integr Plant Biol., 2001, 43(11): 1146-1149.

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Effect of Nitrogen Form on the Activity of Tonoplast Pyrophosphatase in Tomato Roots (in English)

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