J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (5): -.

• Research Articles •    

Construction of a New Bacterial Cloning Vector Using a Mutant Green Fluorescent Protein as an Indicator

DONG Yue-Mei, LI Jiu-Di and ZHU Zhi-Qing   

Abstract: A new bacterial cloning vector, pGreenLD, derived from the triple substitution mutated Aequorea v/ctor/a green fluorescent protein(GFP-S65A, V68L, S72A), when expressed in E. coli produced colonies which showed yellow-green colour under daylight and strong green fluorescence under long-wave ultraviolet light. It can be a useful vector for selecting foreign DNA fragment which was inserted into multiple cloning site based on the loss of the yellow-green color/green fluorescence of E. coli cells attributable to the insertional inactivation of GFP production.

Key words: Green fluorescent protein, GFPmut2, pBluescript SK( +), Cloning vector

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