J Integr Plant Biol. ›› 2007, Vol. 49 ›› Issue (4): 556-567.DOI: 10.1111/j.1744-7909.2007.00442.x

• New Technology • Previous Articles    

High-throughput Binary Vectors for Plant Gene Function Analysis

Zhi-Yong Lei, Ping Zhao, Min-Jie Cao, Rong Cui, Xi Chen, Li-Zhong Xiong, Qi-Fa Zhang, David J. Oliver and Cheng-Bin Xiang   

Abstract: A series of high-throughput binary cloning vectors were constructed to facilitate gene function analysis in higher plants. This vector series consists of plasmids designed for plant expression, promoter analysis, gene silencing, and green fluorescent protein fusions for protein localization. These vectors provide for high-throughput and efficient cloning utilizing sites for l phage integrase/excisionase. In addition, unique restriction sites are incorporated in a multiple cloning site and enable promoter replacement. The entire vector series are available with complete sequence information and detailed annotations and are freely distributed to the scientific community for non-commercial uses.

Key words: binary vectors, gene silencing, green fluorescent protein fusion, high-throughput, promoter analysis, recombination-assisted cloning.

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