J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (2): -.

• Research Articles •    

The Construction of nifH-gfp Expression Vector and Its Expression in Enterobacter gergoviae 57-7

WANG Wei, DONG Yue-Mei, AN Qian-Li, LIU Xiang-Lin, LI Jiu-Di   

Abstract: A nifH-gfp expression vector pMGFP2 was constructed by fusing the 725 bp PCR amplified triple mutated green fluorescent protein (GFP) gene (gfpS65T,V68L,S72A) fragment to the nifH promoter and its start codon which was from Klebsiella pneumoniae (Schr eter) Trevisan M5a1. A kanamycin cassette was inserted into PstⅠ site of pMGFP2, obtaining the expressing vector pMGFP2.1 which can be used for the studying of nifH-gfp expression in Enterobacter gergoviae 57-7. It was then transformed into E.gergoviae 57-7 and the effects of NH+4 and oxygen on the expression of nifH-gfp in E. gergoviae 57-7 were studied.

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