J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (4): -.

• Research Articles •    

Mininucleolus: An Intranuclear Body Enriched with the Splicing Factor SC35

WANG Hua-Chun, XING Miao and ZHANG Chuan-Shan   

Abstract: The mininucleolus (MN) is a distinct intranuclear body often observed in cells of higher plants. Previous studies indicated MN as a non-chromatin structure composed of RNA and proteins, and suggested that it may be involved in the transportation and storage of ribosomal RNA. However, its characteristic protein component and functional significance remain to be clarified. The authors investigated the characteristic protein component of MNs in the nuclei of the meristematic cells of root in Vicia faba L. approaching with an anti- SC35 monoclonal antibody and immunoelectron microscopic observation. A large number of gold particles were observed over the MNs of the specimens labelled with the antibody and protein A-gold, while only a few gold particles were found in the MNs of the control specimens in which labelling of the antibody was omitted. The density of gold particles in MNs of the labelled specimens (300/μm2) was much higher than that of the control specimens (3.76/μm2), demonstrating that SC35 is a constituent of the MN. The identification of the MN highly enriched with the splicing factor will help us understanding the function of this structure.

Key words: Splicing factor SC35, Immunoelectron microscopy, Mininucleolus, Vicia faba

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