J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (10): 912-921.DOI: 10.1111/j.1744-7909.2009.00859.x

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

Actin Dynamics Regulates Voltage-Dependent Calcium-Permeable Channels of the Vicia faba Guard Cell Plasma Membrane

Wei Zhang1,2 and Liu-Min Fan3*   

  1. 1National Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
    2School of Life Sciences, Shandong University, Jinan 250100, China
    3Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
  • Received:2009-03-16 Accepted:2009-06-30 Published:2009-08-21
  • About author:*Author for correspondence Tel(Fax): +86 10 6275 1895; E-mail: lmfan@pku.edu.cn

Abstract:

Free cytosolic Ca2+ ([Ca2+]cyt) is an ubiquitous second messenger in plant cell signaling, and [Ca2+] cyt elevation is associated with Ca2+-permeable channels in the plasma membrane and endomembranes regulated by a wide range of stimuli. However, knowledge regarding Ca2+ channels and their regulation remains limited in planta. A type of voltagedependent Ca2+ permeable channel was identified and characterized for the Vicia faba L. guard cell plasma membrane by using patch-clamp techniques. These channels are permeable to both Ba2+ and Ca2+, and their activities can be inhibited by micromolar Gd3+. The unitary conductance and the reversal potential of the channels depend on the Ca2+ or Ba2+ gradients across the plasma membrane. The inward whole-cell Ca2+ (Ba2+) current, as well as the unitary current amplitude and NPo of the single Ca2+ channel, increase along with the membrane hyperpolarization. Pharmacological experiments suggest that actin dynamics may serve as an upstream regulator of this type of calcium channel of the guard cell plasma membrane. Cytochalasin D, an actin polymerization blocker, activated the NPo of these channels at the single channel level and increased the current amplitude at the whole-cell level. But these channel activations and current increments could be restrained by pretreatment with an F-actin stabilizer, phalloidin. The potential physiological significance of this regulatory mechanism is also discussed.

Zhang W, Fan LM (2009). Actin dynamics regulates voltage-dependent calcium-permeable channels of the Vicia faba guard cell plasma membrane. J. Integr. Plant Biol.51(10), 912-921.

Key words: actin dynamics, calcium-permeable channels, guard cell, patch clamp, plasma membrane, Vicia faba.

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