Production of Artemisinin by Artemisia annua Hairy Root Culture in a Internal Loop Airlift Bioreactor

Abstract

Abstract: A self-made internal loop airlift bioreactor was used for production of artemisinin by Artemisia annua L. hairy root multi-plate culture. The hairy root cultures grew evenly either between the two meshes or attaching to the stainless steel meshes in the bioreactor. Under the optimal conditions of 12 h/d light cycle and 25 ℃, the dry weight and artemisinin yield reached 22.57 g/L and 374.4 mg/L respectively after 20 days. Meantime, the kinetics of sugar, phosphate, nitrate and ammonium uptake during the course of culture were studied.

Key words: Artemisia annua, Internal loop airlift bioreactor, Hairy root, Artemisinin, Kinetics

LIU Chun-Zhao, WANG Yu-Chun, OUYANG Fan, YE He-Chun, and LI Guo-Feng. Production of Artemisinin by Artemisia annua Hairy Root Culture in a Internal Loop Airlift Bioreactor[J]. J Integr Plant Biol., 1999, 41(2): -.

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Production of Artemisinin by Artemisia annua Hairy Root Culture in a Internal Loop Airlift Bioreactor

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