J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (2): -.

• Research Articles •    

Production of Artemisinin by Artemisia annua Hairy Root Culture in a Internal Loop Airlift Bioreactor

LIU Chun-Zhao, WANG Yu-Chun, OUYANG Fan, YE He-Chun, and LI Guo-Feng   

Abstract: A self-made internal loop airlift bioreactor was used for production of artemisinin by Artemisia annua L. hairy root multi-plate culture. The hairy root cultures grew evenly either between the two meshes or attaching to the stainless steel meshes in the bioreactor. Under the optimal conditions of 12 h/d light cycle and 25 ℃, the dry weight and artemisinin yield reached 22.57 g/L and 374.4 mg/L respectively after 20 days. Meantime, the kinetics of sugar, phosphate, nitrate and ammonium uptake during the course of culture were studied.

Key words: Artemisia annua, Internal loop airlift bioreactor, Hairy root, Artemisinin, Kinetics

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