Abstract:

Cell suspension culture of Saussurea medusa Maxim. was established in a 2 L stirred bioreactor for flavonoids production. After 12 days of culture, the biomass was 10.35 g DW·L-1, containing 428.6 mg/L flavonoids, which was 4.14% of the biomass (dry weight) under the condition of 25 ℃ and light intensity lower than 5 μmol·m-2·s-1. The utility pattern of sugar, change of dissolved oxygen and oxygen uptake rate were also studied.

水母雪莲细胞生物反应器悬浮培养
邢建民^1,2 赵德修^2** 叶和春² 李国凤² 李佐虎¹

（1. 中国科学院化工冶金研究所生化工程国家重点实验室，北京100080；2. 中国科学院植物研究所，北京100093）

摘要：利用 2L搅拌式生物反应器进行了水母雪莲 (Saussureamedusa Maxim .)细胞大规模培养。 2 5℃ ,光强低于 5μmol·m-2 ·s-1条件下培养 12d ,生物量为 10 .35gDW·L-1,黄酮产量为 42 8.6mg/L ,达到细胞干重的 4.14%。此外 ,还对培养过程中的糖代谢、溶解氧浓度变化和氧利用速率进行了研究。

关键词： 水母雪莲；细胞培养；黄酮；搅拌式生物反

Key words: Saussurea medusa, suspension culture, flavonoid, bioreactor