J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (11): -.

• Research Articles •    

Variation of Intergeneric Somatic Hybrids Between Citrus sinensis cv. Valencia and Fortunella crassifolia cv. Meiwa

SHI Yong-Zhong,DENG Xiu-Xin and YI Hua-Lin   

Abstract: Intergeneric somatic hybrid plants derived from protoplast fusion between Citrus sinensis (L.) Osbeck cv. Valencia and Fortunella crassifolia Swingle cv. Meiwa have been grown in the fields for 6 years. The plants grew less vigorously with uneven canopy size and some shoots frequently died. Chromosome count showed that the plants, containing non-tetraploid cells besides amphidiploid, were chimeras. Most somatic hybrid plants exhibited a new band of EST isozyme. RAPD analysis verified that several plants lacked some of the parents' markers. The results indicated that the intergeneric somatic hybrids were genetically unstable.

Key words: Citrus, Somatic hybrid, Variation, Random amplified polymorphic DNA

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