J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (10): -.

• Research Articles •    

Ultrastructure of the Vegetative Cells of Nostoc flagelliforme Prepared with High Pressure Freezing and Freeze Substitution Technique

ZHU Jian, WANG Jun, HUA Zhen-Ji and Martin Müller   

Abstract: The ultrastructure of the vegetative cells of Nostoc fiagelliforme Born. et Flah. was investigated with high pressure freezing and freeze substitution technique and compared with the results obtained by using conventional preparation methods. During the processes of chemical fixation, dehydration and embedding, the cell structures might be more artificially modified than that obtained from high pressure freezing and freeze substitution. With the present method, the sheath of N. fiageUiforme could be well-penetrated and no extra big space could exist between the cell and the sheath. The cell protoplasm rarely shrinked. Some fine structures of cell inclusions and unit membranes became visualized. Many bacteria were harbored in the sheath. In addition, the presence of big vacuoles in the cell of N. fiageUiforme as well as the presence of bacteria in the sheath shown in the present preparation for cyanobacteria has not been described so far in the literature.

Key words: Ultrastructure, Nostoc fiagelliforme, Vegetative cell, High pressure freezing, Freeze substitution

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