J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (5): -.

• Research Articles •    

Establishment of Hairy Root Cultures of Astragalus membranaceus and the Extrinsic Factors Affecting Their Growt

HU Zhi-Bi, ZHENG Zhi-Ren, LI Xing-Ping and LIU Di   

Abstract: Hairy root cultures were established from the leaf explant of Astragalus membranaceus (Fisch.) Bge. infected with Agrobacterium rhizogenes and factors that affect their growth were investigated. It was found that the hairy roots grew best in a phytohonnone-free MS medium at pH 6, in which the most optimal concentration of sucrose was 3 % and NH4NO3 was omitted because of its inhibitory effect to the hairy roots. Light was also inhibitory to them but the effect of low temperature was not apparent. Moreover, there were more crude saponin and soluble polysuccharide contents in the hairy roots than in the natural dry roots. Finally, a discussion on the application and the potenrial of the hairy root technique in the production of the plant roots as an effection Chinese traditional medicine was also involved.

Key words: Astragalus membranaceus, Agrobacterium rhizogenes, Hairy roots, In vitro culture, Biologically active compounds

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q