J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (3): -.

• Research Articles •    

Random Amplified Polymorphism of DNA Analysis in Aegilops tauschii

KONG Ling-Rang, DONG Yu-Chen and JIA Ji-Zeng   

Abstract: The genomic DNA variations of 29 accessions of two Aegilops tauschii (Coss.) Schmal. subspeicies from different regions were assayed by random amplified polymorphism of DNA (RAPD) . 136 bands (45.79 % ) behavied polymorphic in 297 RAPD bands amplified by 31 random decamer primers. Of 288 RAPD bands displayed in 20 accessions of ssp. eusquarrosa and 268 bands in 9 accessions of ssp. strangulata, 136 bands (47.22%) and 87 bands (29.29%) were polymorphic respectively, indicating a more divergence of the genomic DNA in the former subspecies than in the latter. In addition, the DNA polymorphism of accessions collected from China was less abundant than that from Iran and former USSR. Genetic distance analysis of 29 accessions based on the 297 RAPD bands revealed that there were two evident clusters in the dendrogram. The accessions of the same subspecies were clustered first. This revealed that the divergence between the two subspecies of Ae. tauschii was greater than that within one subspecies from different geographical regions. The authors also discuss the utilization of Ae. tauschii ssp. eusquarrosa in wheat improvement.

Key words: Aegilops tauschii, RAPD, Diversity

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