J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (5): 452-465.DOI: 10.1111/jipb.12380

• Cell and Developmental Biology • Previous Articles     Next Articles

Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers

Hui Wang1,2,3,4, Pawan Khera2,5, Bingyan Huang6, Mei Yuan4, Ramesh Katam7, Weijian Zhuang8, Karen Harris-Shultz9, Kim M. Moore10, Albert K. Culbreath2, Xinyou Zhang6, Rajeev K. Varshney5, Lianhui Xie1* and Baozhu Guo3*   

  1. 1Fujian Agricultural and Forestry University, College of Plant Protection, Fuzhou, China
    2Department of Plant Pathology, University of Georgia, Tifton, GA, USA
    3USDA-ARS, Crop Protection and Management Research Unit, Tifton, GA, USA
    4Shandong Peanut Research Institute, Qingdao, China
    5International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India
    6Henan Academy of Agricultural Sciences, Cash Crops Research Institute, Zhengzhou, China
    7Department of Biological Sciences, Florida A&M University, Tallahassee, FL, USA
    8Fujian Agricultural and Forestry University, College of Crop Science, Fuzhou, China
    9USDA-ARS, Crop Genetics and Breeding Research Unit, Tifton, GA, USA
    10AgResearch Consultants, Sumner, GA, USA
  • Received:2015-05-25 Accepted:2015-07-13 Published:2015-07-15
  • About author:**Correspondences: (Guo is fully responsible for distributions of all materials associated with this article) E-mail: fjxlh@126.com; baozhu.guo@ars.usda.gov

Abstract:

Cultivated peanut is grown worldwide as rich-source of oil and protein. A broad genetic base is needed for cultivar improvement. The objectives of this study were to develop highly informative simple sequence repeat (SSR) markers and to assess the genetic diversity and population structure of peanut cultivars and breeding lines from different breeding programs in China, India and the US. A total of 111 SSR markers were selected for this study, resulting in a total of 472 alleles. The mean values of gene diversity and polymorphic information content (PIC) were 0.480 and 0.429, respectively. Country-wise analysis revealed that alleles per locus in three countries were similar. The mean gene diversity in the US, China and India was 0.363, 0.489 and 0.47 with an average PIC of 0.323, 0.43 and 0.412, respectively. Genetic analysis using the STRUCTURE divided these peanut lines into two populations (P1, P2), which was consistent with the dendrogram based on genetic distance (G1, G2) and the clustering of principal component analysis. The groupings were related to peanut market types and the geographic origin with a few admixtures. The results could be used by breeding programs to assess the genetic diversity of breeding materials to broaden the genetic base and for molecular genetics studies.

Key words: Arachis hypogaea, breeding, genetic diversity, population structure, simple sequence repeat

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