J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (10): -.

• Research Articles •    

An Insect-Resistant Transgenic Cabbage Plant with Cowpea Trypsin Inhibitor(CpTI) Gene

Fang Hong-jun, Li Da-li, Wang Guan-lin, Li Ying-hui, Zhu Zhen and Li Xiang-hui   

Abstract: Cowpea trypsin inhibitor (CpTI) gene was transformed into Brassica oleracea var. capitara variety "Yingchun" and "Jingfeng" mediated by the Agrobacterium tumefaciens LBA4404(pRCL27). Transgenic plants were obtained from transformed calli or explants. It was shown from the ELISA assay that NPT Ⅱgene was expressed in the transgenic cabbage cells. The integration of the CpTI gene into cabbage genome DNA was confirmed by Southem blotting. Insect-tolerance of the transgenic plants to Pieris rapae L. was observed by bioassays on the transgenic plants in the laboratory.

Key words: Brassica loeracea var. capitata, Cowpea trypsin inhibitor gene, Agrobacterium tumefaciens, Transgenic plants, Insect-resistance

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