Molecular Cloning, Sequencing of Chalcone Isomerase Gene and Its Expression in E. coli

J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (11): -.

• Research Articles •

Molecular Cloning, Sequencing of Chalcone Isomerase Gene and Its Expression in E. coli

Yan Hua, Song Yun, Li Yi-yun, Yan Rui-ming, Li Yi and Chen Zhang-liang

Abstract

Abstract: Anthocyanidins belong to a class of biosynthetic metabolites of flavonoids in higher plants. Chalcone isomerase (CHI) catalyzes the conversion of naringenin chalcone into flavanone. Chi-a gene was cloned from Petunia hybrida Vilm. The sequence of the coding region was 726 bp, encoding a protein of 241 amino acids and was expressed in E. coli. The amino acid sequence homology was compared with CHI from a variety with plant species.

Key words: Chalcone isomerase, Petunia hybrida, Sequence analysis, Expression, Homology comparison

Yan Hua, Song Yun, Li Yi-yun, Yan Rui-ming, Li Yi and Chen Zhang-liang. Molecular Cloning, Sequencing of Chalcone Isomerase Gene and Its Expression in E. coli[J]. J Integr Plant Biol., 1997, 39(11): -.

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Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
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Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号：京ICP备16067583号-22

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号：京ICP备16067583号-22

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Molecular Cloning, Sequencing of Chalcone Isomerase Gene and Its Expression in E. coli

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