J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (11): -.

• Research Articles •    

Molecular Cloning, Sequencing of Chalcone Isomerase Gene and Its Expression in E. coli

Yan Hua, Song Yun, Li Yi-yun, Yan Rui-ming, Li Yi and Chen Zhang-liang   

Abstract: Anthocyanidins belong to a class of biosynthetic metabolites of flavonoids in higher plants. Chalcone isomerase (CHI) catalyzes the conversion of naringenin chalcone into flavanone. Chi-a gene was cloned from Petunia hybrida Vilm. The sequence of the coding region was 726 bp, encoding a protein of 241 amino acids and was expressed in E. coli. The amino acid sequence homology was compared with CHI from a variety with plant species.

Key words: Chalcone isomerase, Petunia hybrida, Sequence analysis, Expression, Homology comparison

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